Biochem/physiol Actions

Reversible: yes

Primary TargetATIC

Cell permeable: yes

General description

A cell-permeable dipeptide that reversibly blocks the homodimerization of aminoimidazole carboxamide ribonucleotide transformylase/inosine nomophosphate cyclohydrolase (ATIC) and inhibits its activity (K_i = 691 nM, K_d = 240 nM). AITC is a bifunctional enzyme that catalyzes the last two steps of de novo purine biosynthesis. Increases the intracellular levels of AICAR leading to the activation of AMP-activated protein kinase (AMPK). Shown to reduce cell viability in a dose-dependent manner. Exhibits a longer half-life and sustained AMPK activation (~ 24 hours) compared to AICA-riboside (Cat. No. 123040).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Spurr, I.B., et al. 2012. ChemBioChem13, 1628.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

Ac-Arg-Ph(4-NO2)-NEt2

Warning

Toxicity: Standard Handling (A)